Antimicrobial Susceptibility-and-Serotypes-of Salmonella Organisms Recovered from Korean Swine

The present study was carried out to investigate serotype prevalence and antimicrobial susceptibility of Salmonella organisms isolated from slaughter pigs in the republic during 3 years periods from 1998 to 2000. A total of 226 cultures of Salmonella spp. were isolated from ileocecal lymph nodes of 1,403 slaughter pigs from 50 farms, of which 42 farms were infected with the organisms. Among 17 serotypes identified the most prevalent serotypes in order of prevalence were S. Typhimurium, S. Reading, S. Derby, S. Agona, S. Enteritidis, S. Schwarzengrund. Other minor serotypes identified were S. Worthington, S. Senftenberg, S. Saintpaul, S. Bolton and S. Mealegridis

Detection of multiresistant Salmonella typhimurium DT104 by multiplex PCR

Phage typing was almost the only way to confirm DT104, but it is so expensive and complicate to perform that it is available in just a few laboratories. Therefore, other rapid and accurate method becomes necessary. PCR has been programmed to detect DTl 04 and known to be very useful. There have been many different PCR programs to identify DT104, but in this study,InvA, Mdh, Pse-1 and ClmA/l\u27etR genes were used to amplify the specific regions of DTl 04 by multiplex PCR, which produce 393, 943, 468 and 602 bp PCR product, respectively. All DT104 were positive to these four genes, and ACSSuT type S. Typhimurium, other Salmonella spec. and other bacteria are negative to the specific genes

Antimicrobial Susceptibility-and-Serotypes-of Salmonella Organisms Recovered from Korean Swine

The present study was carried out to investigate serotype prevalence and antimicrobial susceptibility of Salmonella organisms isolated from slaughter pigs in the republic during 3 years periods from 1998 to 2000. A total of 226 cultures of Salmonella spp. were isolated from ileocecal lymph nodes of 1,403 slaughter pigs from 50 farms, of which 42 farms were infected with the organisms. Among 17 serotypes identified the most prevalent serotypes in order of prevalence were S. Typhimurium, S. Reading, S. Derby, S. Agona, S. Enteritidis, S. Schwarzengrund. Other minor serotypes identified were S. Worthington, S. Senftenberg, S. Saintpaul, S. Bolton and S. Mealegridis.</p

Detection of multiresistant Salmonella typhimurium DT104 by multiplex PCR

Phage typing was almost the only way to confirm DT104, but it is so expensive and complicate to perform that it is available in just a few laboratories. Therefore, other rapid and accurate method becomes necessary. PCR has been programmed to detect DTl 04 and known to be very useful. There have been many different PCR programs to identify DT104, but in this study,InvA, Mdh, Pse-1 and ClmA/l'etR genes were used to amplify the specific regions of DTl 04 by multiplex PCR, which produce 393, 943, 468 and 602 bp PCR product, respectively. All DT104 were positive to these four genes, and ACSSuT type S. Typhimurium, other Salmonella spec. and other bacteria are negative to the specific genes.</p